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ASCB | EMBO San Diego 2018
Annual Meeting of the American Society for Cell Biology and the European Molecular Biology Organization

Dec 08-12, 2018, San Diego, CA, USA
Booth Nr. 1137 (of 89Nort / Chroma Technology)

 

BPS Baltimore 2019
63rd Annual Meeting of the Biophysical Society

Mar 2-6, 2019, Baltimore, Maryland, USA
Booth Nr. 503

 

NWG Göttingen 2019
13th Meeting of the German Neuroscience Society

Mar 20-23, 2019, Göttingen, Germany
Booth Nr. 8

Photobleaching / FRAP

Accurate Photobleaching

Exchange dynamics of HP-1, a protein located in the centromere, were analyzed by using the FRAP (fluorescence recovery after photobleaching) technique. For this, Dendra-2 was fused to the HP-1 protein. For photobleaching experiments an UGA-40 point scanning device in combination with a 405 nm diode laser (DL-405/100) was used. Due to the high accuracy of the system, FRAP experiments of single protein aggregates could be performed, without affecting neighboring structures.

 

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Investigation of Protein Dynamics in Nerve Terminals Using FRAP

To investigate the dynamics of endogenous Munc18-1 – a protein involved in fusion of synaptic vesicle – FRAP (fluorescence recovery after photobleaching) experiments were performed. To this end using mice expressing fluorescently labeled Munc18-1 from the endogenous munc18-1 locus were used.

 

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