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ASCB | EMBO San Diego 2018
Annual Meeting of the American Society for Cell Biology and the European Molecular Biology Organization

Dec 08-12, 2018, San Diego, CA, USA
Booth Nr. 1137 (of 89Nort / Chroma Technology)

 

BPS Baltimore 2019
63rd Annual Meeting of the Biophysical Society

Mar 2-6, 2019, Baltimore, Maryland, USA
Booth Nr. 503

 

NWG Göttingen 2019
13th Meeting of the German Neuroscience Society

Mar 20-23, 2019, Göttingen, Germany
Booth Nr. 8

Localized Glutamate Uncaging in CA1 Pyramidal Neurons in Brain Slices

EPSCs (Excitatory Post Synaptic Currents) obtained in whole-cell voltage clamp mode in response to focal uncaging of glutamate onto multiple locations (marked by red crosses) around a single dendritic branch of a CA1 pyramidal neuron. Neurons were loaded with bis-Fura-2 by intracellular injection in a brain slice. The release zone is highly localized as evident from the relationship between the signal amplitude and the distance of the uncaging spot from the dendrite. (Uncaging in seven spots between the arrow heads)

 

Equipment:

 

  • Rapp OptoElectronic UGA-40 spot illumination system with DL-405/100 laser on Zeiss AxioObserver, 63x water immersion objective, RedShirt Imaging camera.
  • Spot size <1µ.
  • Synchronization via Master8 TTL protocols
Excitatory Post Synaptic Currents evoked by localized Glu uncaging in and near dendrites

Data courtesy of Dr. Marko Popovic, Laboratory of Dr. Dejan Zecevic, Dept. of Cellular & Molecular Physiology, Yale School of Medicine, New Haven, USA.

 
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