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ASCB | EMBO San Diego 2018
Annual Meeting of the American Society for Cell Biology and the European Molecular Biology Organization

Dec 08-12, 2018, San Diego, CA, USA
Booth Nr. 1137 (of 89Nort / Chroma Technology)

 

BPS Baltimore 2019
63rd Annual Meeting of the Biophysical Society

Mar 2-6, 2019, Baltimore, Maryland, USA
Booth Nr. 503

 

NWG Göttingen 2019
13th Meeting of the German Neuroscience Society

Mar 20-23, 2019, Göttingen, Germany
Booth Nr. 8

UGA-42-Firefly - Point Scanning Device

The UGA-42 Firefly is a galvanometer based movable spot system designed for the illumination of small spots in the field of view of the microscope. The use of scanners allows the fast relocation of the spot anywhere in the field as well as the scanning of larger regions of interest (ROI).
The UGA-42 Firefly includes the scanner module, the optical and mechanical components necessary to create the spot and to attach the module to the microscope, the microcontroller and the software. The optical module is coupled to the microscope without interfering with most other existing devices (i.e. spinning disk, LSM, etc.). Standard systems provide coupling for one or two fiber coupled lasers, but options for up to four lasers are available. The lasers are application specific, have to be ordered separately. Please visit the LASER SYSTEMS page for more information.

THE UGA-42 FIREFLY AT A GLANCE

Scanner module:

  • Add-on module, can be integrated into existing set-ups
  • Switching time between any two spot locations <1ms
  • Scanning speed within an ROI up to 5kHz
  • Spot size as low as submicron, depending on microscope, objective and light guide aperture
  • Wide variety of  UV, VIS and NIR lasers available (266 to 1470 nm)
  • Up to four lasers per system
  • Switching time between two laser lines: <1ms
  • Laser coupling to the system: direct or via light guide
  • Can be combined with wide field fluorescence, LSM and spinning disc confocal, etc.
  • Modules available for most modern research microscope brands and models

Please contact us for more information

SysCon Software:

  • The SysCon software runs in parallel with and independent of any other imaging or electrophysiology software
  • Simultaneous and independent spot illumination and imaging data acquisition
  • “Click & Fire” mode for real time spot illumination 
  • Programmable illumination (sequences) of multiple locations and/or regions of interests (ROIs)
  • ROI geometries include: lines, circles, polygons, free-hand drawn shapes
  • Grid stimulation with sequential or random illumination of grid points
  • TTL-in and -out 
  • Manual or TTL-triggered sequence start
  • TTL-triggered events within the sequence
  • Able to run up to two lasers within the same experiment

Applications

  • Ablation, cutting, microdissection
  • Photobleaching and FRAP
  • Photolysis, uncaging, drug photorelease
  • Optogenetics
  • Photoactivation
  • Photoswitching
  • Photoconvertion
  • Photostimulation
  • Neural mapping, dynamics and plasticity
  • FRET
Kohl, M. M. et al., (2009). Cell type specific optical stimulation reveals left-right asymmetry of hippocampal plasticity, poster, SFN Meeting
 
Rapp OptoElectronic GmbH  |  Managing Director: Dr. Gert Rapp · Kronskamp 110 · 22880 Wedel · GERMANY · Phone +49 (0)4103 701 890 · Fax +49 (0)4103-701 8921 © 2013 s. Legal notice