Search


News / Meetings

VISIT US AT:

 

European Light Microscopy Initiative

17th International ELMI Meeting

Booth 15

May 23-26, Dubrovnik, Croatia

 

Society for Neuroscience (SfN) 2017 Annual Meeting

Booth 2610

November 11-15, Washington DC, USA

 

Uncaging

Calcium rises evoked by localized photolysis of caged IP3

The smooth muscle cell was voltage clamped in whole cell configuration and the membrane potential maintained at -70mV. Calcium was imaged using fluo-3 at a rate of 25 frames per second and released from the internal calcium store by photolyzing caged IP3. Caged IP3 was introduced to the cell via the patch pipette filling solution. Contraction was prevented by the drug Wortmanin.

 

DETAILS...

Imaging Sodium Transients as a Response to Local Uncaging of MNI-Glutamate

Whole-cell patch-clamp, multi-photon sodium imaging and (UV)-light-induced uncaging of glutamate were combined to investigate postsynaptic sodium signals in cellular micro domains of central neurons. In detail, whole-cell recordings were performed on Cornu Ammonis subdivision 1 (CA1) pyramidal neurons in acute tissue slices. In addition, neurons were....

 

DETAILS...

Localized Glutamate Uncaging in CA1 Pyramidal Neurons in Brain Slices

EPSCs (Excitatory Post Synaptic Currents) obtained in whole-cell voltage clamp mode in response to focal uncaging of glutamate onto multiple locations (marked by red crosses) around a single dendritic branch of a CA1 pyramidal neuron. Neurons were loaded with bis-Fura-2 by intracellular injection in a brain slice. The release zone is highly localized as evident from the relationship between the signal amplitude and the distance of the uncaging spot from the dendrite.

 

DETAILS...

Investigation of the Interglomerular Lateral Inhibition in Rat Olfactory Bulb

The study investigated the presence and the mechanism of lateral inhibition between glomeruli in the rat olfactory bulb. Therefore, slices of olfactory bulb were prepared to analyze the inhibitory effects of nearby glomeruli. As control, the olfactory sensory neurons in a glomerulus were stimulated by an electrical stimulus. Afterwards, nearby glomeruli were stimulated by photoactivated glutamate release, using a 355 nm DPSL-355/30 laser (Rapp OptoElectronic) coupled to a Rapp OptoElectronic UGA-40 scanner based point illumination system.

 

DETAILS...

Laser Photolysis of Caged Calcium (NP-EGTA)

Hippocampal slices of P16 mice were stained with the cell-permeant calcium-sensitive dye Fluo-4 AM and additionally loaded with cell permeant NP-caged EGTA AM. This photolabile chelator releases Ca2+ upon UV illumination due to its Kd increase from 80 nM to >1 mM. Uncaging of calcium was achieved by the application of brief (500 ms) and focally restricted flashes of UV light (diameter ~1 μm) to astrocytic somata during two-photon-imaging of these cells.

 

DETAILS...

Calcium uncaging - Light induced stimulation of hippocampal neurons

Recent improvements in light microscopic techniques, in particular laser-scanning fluorescence microscopy, in combination with optical stimulation methods, namely optical switches and caged molecules, have made it possible to study the structure and function of neurons and their synapses in intact brain tissue with high spatial and temporal resolution. These novel techniques are increasingly making it possible to study the neuronal mechanisms underlying brain functions such as learning and memory.

 

DETAILS...

Response of Isolated Olfactory Receptor Neurons from Ano2+/+ and Ano2-/- Mice to Photoreleased Ca2+ or 8-Br-cAMP

Typical current responses to a sudden [Ca2+] increase in isolated olfactory sensory neurons (OSNs) from Ano2+/+ and Ano2-/- mice in whole-cell patch-clamp experiments under symmetrical Cl-. Cells were clamped  to -50 mV, 0 mV and +50 mV. Arrows indicate the flash that releases Ca2+ from DMNP-EDTA. Inset, larger magnification to reveal the currents remaining in Ano2-/- OSNs.

 

DETAILS...

 
Rapp OptoElectronic GmbH  |  Managing Director: Dr. Gert Rapp · Kronskamp 110 · 22880 Wedel · GERMANY · Phone +49 (0)4103 701 890 · Fax +49 (0)4103-701 8921 © 2013 s. Legal notice