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ASCB | EMBO San Diego 2018
Annual Meeting of the American Society for Cell Biology and the European Molecular Biology Organization

Dec 08-12, 2018, San Diego, CA, USA
Booth Nr. 1137 (of 89Nort / Chroma Technology)

 

BPS Baltimore 2019
63rd Annual Meeting of the Biophysical Society

Mar 2-6, 2019, Baltimore, Maryland, USA
Booth Nr. 503

 

NWG Göttingen 2019
13th Meeting of the German Neuroscience Society

Mar 20-23, 2019, Göttingen, Germany
Booth Nr. 8

DNA Damage and Repair Mechanisms

Equipment

  • UGA-40-MLC ablation system with DPSL-355/14, DL-405, DL-473 and DPSL-543 lasers
  • Zeiss AxioObserver Z1 with Spinning Disc unit and four-line laser combiner, AxioCam and Photometrics Evolve cameras (AxioCam used in the experiment)
  • Objective 63x water immersion
UGA-40-MLC ablation system on Zeiss AxioObesrver with SD unit - Zeiss Demo Center, Munich Germany

Experimental information

  • GFP imaging at 488 nm, in spinning disc mode using ZEN Blue software from Zeiss.
  • DNA damage induced by UV ablation at 355 nm. Repair proteins tagged with GFP. As they gather in the damaged areas, the local fluorescence increases and the spots appear brighter in the image.
  • Experiment set up in a high throughput style, without prescreening, in a multiwell plate.
  • Photomanipulation software run in Grid mode. A multi point grid was set up to be run in each well while the sample was continuously imaged. The DNA damage was induced in the nuclei which happened to be located in the light path. The damage locations became more fluorescent during the imaging due to the accumulation of the GFP-tagged repair proteins.

Data courtesy of Dr. Gyula Timinszky, Ludwig Maximilian University, Munich, Germany. Acquired during the demonstration of the Zeiss AxioObserver SD Confocal/Ablation combination system at the Zeiss Demonstration Center in Munich .

 
Rapp OptoElectronic GmbH  |  Managing Director: Dr. Gert Rapp · Kronskamp 110 · 22880 Wedel · GERMANY · Phone +49 (0)4103 701 890 · Fax +49 (0)4103-701 8921 © 2013 s. Legal notice