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ASCB | EMBO San Diego 2018
Annual Meeting of the American Society for Cell Biology and the European Molecular Biology Organization

Dec 08-12, 2018, San Diego, CA, USA
Booth Nr. 1137 (of 89Nort / Chroma Technology)

 

BPS Baltimore 2019
63rd Annual Meeting of the Biophysical Society

Mar 2-6, 2019, Baltimore, Maryland, USA
Booth Nr. 503

 

NWG Göttingen 2019
13th Meeting of the German Neuroscience Society

Mar 20-23, 2019, Göttingen, Germany
Booth Nr. 8

Calcium rises evoked by localized photolysis of caged IP3

Single smooth muscle cell

The smooth muscle cell was voltage clamped in whole cell configuration and the membrane potential maintained at -70mV. Calcium was imaged using fluo-3 at a rate of 25 frames per second and released from the internal calcium store by photolyzing caged IP3. Caged IP3 was introduced to the cell via the patch pipette filling solution. Contraction was prevented by the drug Wortmanin.

Uncaging spots

 

Using the  UGA-40 system for a fast movable Spot Illumination a sequence of 5 spots to be illuminated has been programmed. The delay time between the illuminated spots was 1 sec each, the power of the laser (DPSL 355/30) has been reduced to less than 10%, the pulse was 5ms long. The spots to be illuminated were less than 4 µm and are marked with a red cross in the image below. The calcium response may be watched in the movie below.

Data by courtesy of:
Prof. John McCarron
Strathclyde Institute of Pharmacy and Biomedical Sciences (SIPBS)
27 Taylor Street
Glasgow, U.K.

 
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