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VISIT US AT:

 

ASCB | EMBO San Diego 2018
Annual Meeting of the American Society for Cell Biology and the European Molecular Biology Organization

Dec 08-12, 2018, San Diego, CA, USA
Booth Nr. 1137 (of 89Nort / Chroma Technology)

 

BPS Baltimore 2019
63rd Annual Meeting of the Biophysical Society

Mar 2-6, 2019, Baltimore, Maryland, USA
Booth Nr. 503

 

NWG Göttingen 2019
13th Meeting of the German Neuroscience Society

Mar 20-23, 2019, Göttingen, Germany
Booth Nr. 8

IBIXX-1 Upright and Inverted Hybrid Microscope

The IBIXX-1 is a multi-functional, multi-purpose microscope for double-sided sample illumination and observation

IBIXX-1 with two UGA-40 modules

Based on the Olympus BX and IX series microscopes, the IBIXX-1 provides the user with unprecedented versatility in configuring scientific experiments using a variety of methods. The microscope provides two ports for upright and inverted full field illumination in transmitted light, as well as in fluorecence. By using objectives with different NA, the sizes of the fields of view illuminated from above and from below can be different, providing the general view of a large portion of the sample from one side (for instance a whole cultured neuron), while allowing the detailed view of a smaller area from the other side (for instance a dendrite branch)

IBIXX-1 with µ-Matrix module on the upright path

A new level of functionality can be added by using the IBIXX-1 with the Rapp Opto spot and pattern illumination devices, the UGA-42 Firefly and UGA-42-GEO, the µ-Matrix and the AiWon. Now full field imaging from one side of the sample can integrated with localized photostimulation (eg. photoactivation or uncaging) from the other side, allowing the study of the signal transmission across thick samples without the need of expensive 2-photon stimulation to avoid unwanted signals from the intermediate layers.

Plasmonic device - IIT, SISSA, Italy

The flexibility and versatility of the IBIXX-1 microscope makes it a powerful tool in:

  • electrophysiology in both patch clamp techniques and recordings using multielectrode arrays
  • single molecule photoactivation in conjunction with plasmonic hole arrays
  • retina stimulation studies

IBIXX-1 AT A GLANCE

  • Upright and inverted combination microscope
  • Full field illumination from both sides of the sample in both transmitted light and fluorescence
  • Easily integrated with spot and pattern illumination devices
  • Photomanipulation possible from above and below the sample in parallel with imaging or electrophysiology

APPLICATIONS

When combined with any of the spot or pattern illumination systems, the IBIXX can be used in any of the applications specific to the respective devices. In addition:

 

MEA recording, group of Dr. Zeck, NMI Reutlingen

1. In combination with plasmonic hole arrays: single cell photoactivation in cell cultured on the plasmonic cell arrays

 

2. In combination with any of the spot and pattern illumination devices: mapping, connectivity, signal transduction across the thickness of the tissue by photostimulating (photoactivation/inhibition, uncaging, optogenetics, photo stimulation of retina) on one side of the sample and imaging or recording (patch-clamp, MEA) on the other.

PHOTOMANIPULATION OPTIONS

1. Single sided photomanipulation

  • One fixed spot (AiWon) or scanner based (UGA-42 Firefly or UGA-42 GEO) system coupled either into the upright or to the inverted epifluorescence path
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  • One pattern illumination system coupled above the objective of the upright epi-iluminator

2. Double sided photomanipulation

  • Two spot illumination systems, any combination, coupled one into each epifluorescence path
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  • One spot illumination system (AiWon, UGA-42 Firefly or UGA-42 GEO) coupled into the inverted epifluorescence path and one pattern illumination system (µ-Matrix) coupled above the objective of the upright epi-iluminator

 

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Rapp OptoElectronic GmbH  |  Managing Director: Dr. Gert Rapp · Kronskamp 110 · 22880 Wedel · GERMANY · Phone +49 (0)4103 701 890 · Fax +49 (0)4103-701 8921 © 2013 s. Legal notice