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NWG Göttingen 2019
13th meeting of the German Neuroscience Society

Mar 20-23, 2019, Göttingen, Germany
Booth No. 8

 

SFN - Neuroscience 2019
50th annual meeting of the Society for Neuroscience

Oct 19-23, 2019, Chicago, IL, USA
Booth No. 1468

 

BPS San Diego 2020
64th annual meeting of the Biophysical Society

Feb 15-19, 2020 San Diego, CA, USA
Booth No. 710

Photobleaching / FRAP

Accurate Photobleaching

Exchange dynamics of HP-1, a protein located in the centromere, were analyzed by using the FRAP (fluorescence recovery after photobleaching) technique. For this, Dendra-2 was fused to the HP-1 protein. For photobleaching experiments an UGA-40 point scanning device in combination with a 405 nm diode laser (DL-405/100) was used. Due to the high accuracy of the system, FRAP experiments of single protein aggregates could be performed, without affecting neighboring structures.

 

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Investigation of Protein Dynamics in Nerve Terminals Using FRAP

To investigate the dynamics of endogenous Munc18-1 – a protein involved in fusion of synaptic vesicle – FRAP (fluorescence recovery after photobleaching) experiments were performed. To this end using mice expressing fluorescently labeled Munc18-1 from the endogenous munc18-1 locus were used.

 

DETAILS...

 
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