News / Meetings



ASCB | EMBO San Diego 2018
Annual Meeting of the American Society for Cell Biology and the European Molecular Biology Organization

Dec 08-12, 2018, San Diego, CA, USA
Booth Nr. 1137 (of 89Nort / Chroma Technology)


BPS Baltimore 2019
63rd Annual Meeting of the Biophysical Society

Mar 2-6, 2019, Baltimore, Maryland, USA
Booth Nr. 503


NWG Göttingen 2019
13th Meeting of the German Neuroscience Society

Mar 20-23, 2019, Göttingen, Germany
Booth Nr. 8

Photobleaching / FRAP

Accurate Photobleaching

Exchange dynamics of HP-1, a protein located in the centromere, were analyzed by using the FRAP (fluorescence recovery after photobleaching) technique. For this, Dendra-2 was fused to the HP-1 protein. For photobleaching experiments an UGA-40 point scanning device in combination with a 405 nm diode laser (DL-405/100) was used. Due to the high accuracy of the system, FRAP experiments of single protein aggregates could be performed, without affecting neighboring structures.



Investigation of Protein Dynamics in Nerve Terminals Using FRAP

To investigate the dynamics of endogenous Munc18-1 – a protein involved in fusion of synaptic vesicle – FRAP (fluorescence recovery after photobleaching) experiments were performed. To this end using mice expressing fluorescently labeled Munc18-1 from the endogenous munc18-1 locus were used.



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