News / Meetings



NWG Göttingen 2019
13th meeting of the German Neuroscience Society

Mar 20-23, 2019, Göttingen, Germany
Booth No. 8


SFN - Neuroscience 2019
50th annual meeting of the Society for Neuroscience

Oct 19-23, 2019, Chicago, IL, USA
Booth No. 1468


BPS San Diego 2020
64th annual meeting of the Biophysical Society

Feb 15-19, 2020 San Diego, CA, USA
Booth No. 710

Photobleaching / FRAP

Accurate Photobleaching

Exchange dynamics of HP-1, a protein located in the centromere, were analyzed by using the FRAP (fluorescence recovery after photobleaching) technique. For this, Dendra-2 was fused to the HP-1 protein. For photobleaching experiments an UGA-40 point scanning device in combination with a 405 nm diode laser (DL-405/100) was used. Due to the high accuracy of the system, FRAP experiments of single protein aggregates could be performed, without affecting neighboring structures.



Investigation of Protein Dynamics in Nerve Terminals Using FRAP

To investigate the dynamics of endogenous Munc18-1 – a protein involved in fusion of synaptic vesicle – FRAP (fluorescence recovery after photobleaching) experiments were performed. To this end using mice expressing fluorescently labeled Munc18-1 from the endogenous munc18-1 locus were used.



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