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NWG Göttingen 2019
13th meeting of the German Neuroscience Society

Mar 20-23, 2019, Göttingen, Germany
Booth No. 8

 

SFN - Neuroscience 2019
50th annual meeting of the Society for Neuroscience

Oct 19-23, 2019, Chicago, IL, USA
Booth No. 1468

 

BPS San Diego 2020
64th annual meeting of the Biophysical Society

Feb 15-19, 2020 San Diego, CA, USA
Booth No. 710

DNA Damage and Repair Mechanisms

Equipment

  • UGA-40-MLC ablation system with DPSL-355/14, DL-405, DL-473 and DPSL-543 lasers
  • Zeiss AxioObserver Z1 with Spinning Disc unit and four-line laser combiner, AxioCam and Photometrics Evolve cameras (AxioCam used in the experiment)
  • Objective 63x water immersion
UGA-40-MLC ablation system on Zeiss AxioObesrver with SD unit - Zeiss Demo Center, Munich Germany

Experimental information

  • GFP imaging at 488 nm, in spinning disc mode using ZEN Blue software from Zeiss.
  • DNA damage induced by UV ablation at 355 nm. Repair proteins tagged with GFP. As they gather in the damaged areas, the local fluorescence increases and the spots appear brighter in the image.
  • Experiment set up in a high throughput style, without prescreening, in a multiwell plate.
  • Photomanipulation software run in Grid mode. A multi point grid was set up to be run in each well while the sample was continuously imaged. The DNA damage was induced in the nuclei which happened to be located in the light path. The damage locations became more fluorescent during the imaging due to the accumulation of the GFP-tagged repair proteins.

Data courtesy of Dr. Gyula Timinszky, Ludwig Maximilian University, Munich, Germany. Acquired during the demonstration of the Zeiss AxioObserver SD Confocal/Ablation combination system at the Zeiss Demonstration Center in Munich .

 
Rapp OptoElectronic GmbH  |  Managing Director: Dr. Gert Rapp · Kronskamp 110 · 22880 Wedel · GERMANY · Phone +49 (0)4103 701 890 · Fax +49 (0)4103-701 8921 © 2013 s. Legal notice