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SP-20

High Energy Flash Lamp System (µs Pulse)

The SP-20 is a light guide coupled, tunable and single pulsed flash lamp system featuring a high pulse energy and quartz optics. Compared to the JML-C2, the system offers a pulse length from 4 to 300 µs at the full width half maximum (FWHM) depending on the settings. Thus, the SP-20 is the perfect flash lamp solution for all applications where a microsecond light pulse with high energy is required.

Features

Flexibility

The SP-20 flash lamp system can be adapted to almost all models of major research microscope brands. In general, two kinds of illumination are possible based on the light guide and the type of adaptation. Coupling by a liquid light guide adapter (LGA) gives the possibility to illuminate the whole field of view. In contrast, a spot illumination module (AiWon) enables the user to illuminat a quite small area of the field of view with a high energy light pulse.

Easy Control and Adjustment

This pulsed light source system can be triggered externally by an optical isolated trigger signal to avoid any disturbance of the light flash. The energy level is adjustable via the capacitor settings. To define a specific spectral output based on your experimental needs, up to two optical filters can be placed between the bulb and the light guide.

Applications
  • Flash Uncaging
  • Flash Photolysis
  • Flash Excitation
  • Illumination
Specifications
Compatibility: Adapter for most research microscope models available
Light guide:Broad range available (100 µm – 10 mm in diameter)
Bulb: Xenon point source flash bulb
Wavelength: 250-1000 nm (filter selectable spectral output)
Optical filter:Two slots for 25 mm (1‘‘) filter
Pulse energy:Up to 140 J/ pulse (adjustable)
Pulse length:4-300 μs (FWHM), depending on capacitors settings
Pulse repetition: 50 Hz (high repetition rate)
Trigger:Manual, external, optical isolated
Line Voltage115-230 V
Frequency:50-60 Hz
Dimensions (W x D x H):450 x 300 x 135 mm
Weight:Approx. 14 kg
Delivery content:1x flash lamp system, 1x light guide, 1x UV-bandpass-filter
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Literature
  • Kochubey, Olexiy, Yunyun Han, and Ralf Schneggenburger. 2009. “Developmental Regulation of the Intracellular Ca2+ Sensitivity of Vesicle Fusion and Ca2+-Secretion Coupling at the Rat Calyx of Held.” The Journal of Physiology 587(Pt 12):3009–23.
  • Korogod, Natalya, Xuelin Lou, and Ralf Schneggenburger. 2007. “Posttetanic Potentiation Critically Depends on an Enhanced Ca(2+) Sensitivity of Vesicle Fusion Mediated by Presynaptic PKC.” Proceedings of the National Academy of Sciences of the United States of America 104(40):15923–28.
  • Wölfel, Markus, Xuelin Lou, and Ralf Schneggenburger. 2007. “A Mechanism Intrinsic to the Vesicle Fusion Machinery Determines Fast and Slow Transmitter Release at a Large CNS Synapse.” The Journal of Neuroscience : The Official Journal of the Society for Neuroscience 27(12):3198–3210.
  • Lou, Xuelin, Volker Scheuss, and Ralf Schneggenburger. 2005. “Allosteric Modulation of the Presynaptic Ca2+ Sensor for Vesicle Fusion.” Nature 435(7041):497–501.
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