The AiWon is a fixed spot illumination device leading to a fixed spot position in the middle of the field of view. It is designed for applications which require neither rapid switching nor simultaneous illumination of different regions. It is the basic system of our AiWon series and it is especially designed to illuminate a small spot with high power density. The spot size depends on the objective and the light guide used.
The AiWon can be easily combined and upgraded with additional ports for light sources or other modules of the AiWon series such as the AiWon Geo selectable shape scanning module and/ or the AiWon Caliburn laser ablation module. It makes the AiWon the ideal solution for applications where a fixed-spot illumination is sufficient. Furthermore, the AiWon together with other devices of the AiWon series can be connected to all models of major research microscope brands, giving you the possibility to choose the perfect microscope for your applications and individual needs.
Light Source Flexibility
The AiWon provides optical and mechanical components to couple the light source into the microscope and to produce the desired spot size. The module will be added to the fluorescence beam path without requiring any modification of the microscope. Suitable light sources are laser systems, LEDs or flash lamps depending on the experimental requirements (wavelength, power density, spot size, etc.). The light source is coupled to the device via an optical fiber or liquid light guide, but certain applications like ablation, microdissection and DNA-damage may require direct coupling.
- Photoactivation/ Photoinhibition
- Photoconversion/ Photoswitching
- Photobleaching/ FRAP
- Uncaging/ Photolysis
- Optogenetics/ Photostimulation
- Neural mapping, dynamics and plasticity
Bendels, Michael H. K., Prateep Beed, Christian Leibold, Dietmar Schmitz, and Friedrich W. Johenning. 2008. “A Novel Control Software That Improves the Experimental Workflow of Scanning Photostimulation Experiments.” Journal of Neuroscience Methods 175(1):44–57.
Weiskopf, Daniela, Eva K. Schmitt, Marco H. Klühr, Stephan K. Dertinger, and Claudia Steinem. 2007. “Micro-BLMs on Highly Ordered Porous Silicon Substrates: Rupture Process and Lateral Mobility.” Langmuir : The ACS Journal of Surfaces and Colloids 23(18):9134–39.
Sobie, Eric A., Joseph P. Y. Kao, and W. J. Lederer. 2007. “Novel Approach to Real-Time Flash Photolysis and Confocal [Ca2+] Imaging.” Pflügers Archiv : European Journal of Physiology 454(4):663–73.