The UVILED is a high power fiber coupled LED system covering 365 nm for photolysis, fluorescence excitation, uncaging, etc.. The system can be operated continuously or pulsed with a high peak power. The standard quartz or liquid light guide can be adapted to each microscope of major brands, it can also be used for customized setups suiting to your application. The remote control allows an easy handling of the UVILED while sitting in the front of your microscope. In addition, the built-in mechanical shutter gives you the possibility to control the illumination of your samples easily and precisely by opening and closing the shutter with stable and high LED output power.
Different Light Guide, Different Output Power
The used type of the light guide for the UVILED influences the output power at the light guide exit.
The parameters based on the light guide are:
- 3 mm light guide:
- ~ 540 mW (pulse length 10 ms, duty cycle 10%)
- ~ 200 mW continuous
- 1250 µm quartz light guide:
- ~ 100 mW (pulse length 10 ms, duty cycle 10%)
- ~ 37 mW continuous
The UVILED is not only a UV-light source used for microscopic applications. It can also be used as well for UV curing. Therefore, we offer the UVILED‑T including the UV-light source, a special handheld to get close to the object and all other features of the standard UVILED. At an output power of more than 70 mW the object can be illuminated with 10 mW/cm² at a distance of 20 mm. Optional, the UVILED-T is available with a refocusing optic. A warm-up is not required.
- UV illumination
- Fluorescence excitation
- UV curing
- High power UV LED system
- Wavelength 365 nm
- Continuous or pulsed operation with high peak power
- Remote control
- Built-in mechanical shutter
- Complete system with quartz or liquid light guide coupling to the microscope
- Power >70mW
- ~10mW/cm2 at 20mm distance
Miyazaki, K. and W. N. Ross. 2013. “Ca2+ Sparks and Puffs Are Generated and Interact in Rat Hippocampal CA1 Pyramidal Neuron Dendrites.” Journal of Neuroscience 33(45):17777–88.
Umeda, Nobuhiro, Tasuku Ueno, Christopher Pohlmeyer, Tetsuo Nagano, and Takanari Inoue. 2011. “A Photocleavable Rapamycin Conjugate for Spatiotemporal Control of Small GTPase Activity.” Journal of the American Chemical Society 133(1):12–14.
Manita, Satoshi, Kenichi Miyazaki, and William N. Ross. 2011. “Synaptically Activated Ca2+ Waves and NMDA Spikes Locally Suppress Voltage-Dependent Ca2+ Signalling in Rat Pyramidal Cell Dendrites.” The Journal of Physiology 589(Pt 20):4903–20.
Hong, Min and William N. Ross. 2007. “Priming of Intracellular Calcium Stores in Rat CA1 Pyramidal Neurons.” The Journal of Physiology 584(Pt 1):75–87.