OptoSplit II BP
Dual Emission Image Splitter with Bypass
Cairn Research Ltd.
The dual emission image splitter OptoSplit II Bypass (BP) from our partner company Cairn Research Ltd. is the improved dual channel simultaneous imaging device for use with a single camera. It builds on the success of the OptoSplit II, but adds a convenient single lever bypass mode making it more suitable for multi-user microscopes where simultaneous dual channels are only required for specific experiments alongside single wavelength recordings. In addition, the simple and accessible design makes the OptoSplit II BP an excellent platform for alternative applications, such as dual polarization imaging.
High-End Dual Image Splitter
Whilst maintaining compatibility with the OptoSplit II, the bypass version now supports our new flat-face filter cubes and has enhanced long-term stability, pixel registration and reproducibility. Feature wise, the rapid bypass control is complimented by additional space for more auxiliary components. It has a slightly larger footprint than the OptoSplit II and consequently, can use longer focal ratio lenses with even better off-axis performance. Optimized for coupling to a scientific microscope, the OptoSplit II BP can also be used with camera lenses or any other system of lenses which produce an image plane of suitable size and f/number.
Device drivers are included in most commercial imaging packages to assist registration and to allow real-time and off-line ratioing of fluorescence overlays. Alternatively, the OptoSplit II BP can be used with simple image capture software, the processing carried out manually offline or by using our own MicroManager and ImageJ drivers.