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UGA-42 Firefly

Point Scanning Device for Localized Photomanipulation

The UGA-42 Firefly is one of the scanner-based systems of our UGA-42 series for localized photomanipulation. It is the basic system of this product range and is especially designed to illuminate small spots or larger regions of interest (ROIs) with high power density. Thus, the UGA-42 Firefly is the perfect scanner solution for all applications where high laser intensity is required.

Features

Modularity

The UGA‑42 Firefly can be easily combined and upgraded with up to four separate laser ports and/ or with all other modules of the UGA‑42 series such as the UGA‑42 Geo selectable spot shape scanning module and/ or the UGA‑42 Caliburn laser ablation module. This modularity makes the UGA‑42 Firefly the ideal system to start with. Furthermore, UGA‑42 devices can be connected to almost all models of major research microscope brands, giving you the possibility to choose the perfect microscope for your applications and individual needs.

High Flexibility

With the intuitive and easy-to-use SysCon software one can generate a diversity of shapes, sizes, customization and grid pattern options to define a region of interest (ROI). The “Click and Fire” mode allows photomanipulation of e.g. moving objects. Different plugins for the SysCon software are available for direct communication with standard imaging software packages.

Precision and Speed

Due to the real-time controller the scan-mirrors of the UGA‑42 Firefly are extremely precise both spatially and temporarily. Based on the setup in the SysCon sequence manger, the laser beam moves accurately and fast across the sample. Easy hardware synchronization with other devices via TTL ports gives you even more possibilities to setup precise and complex experiments by using auxiliary devices.

Applications
  • Photoactivation/ Photoinhibition
  • Photoconversion/ Photoswitching
  • Photoexcitation
  • Photobleaching/ FRAP
  • Uncaging/ Photostimulation
  • Optogenetics/ Photolysis
  • Neural mapping, dynamics and plasticity
Downloads

For microscopes:

  • Flyer Photomanipulation (3.5 MB)

For macroscopes:

  • Flyer UGA-42 series for Olympus MVX10 (1.1 MB)
  • Flyer UGA-42 series for Zeiss AxioZoom.V16 (1.1 MB)

SysCon Software:

  • Flyer SysCon & ZEN Communication (1.1 MB)
  • Flyer SysCon & NIS Elements Communication (1.1 MB)
  • Flyer SysCon & MicroManager Communication (1.1 MB)
  • Flyer SysCon & MetaMorph Communication (1.1 MB)
  • Flyer SysCon Software & Communication Plugin (1.2 MB)
  • Flyer SysCon & cellSens Communication (1.2 MB)
Literature

Huet, Sébastien, Gyula Timinszky, Rebecca Smith, Hafida Sellou, and Catherine Chapuis. 2018. “CHD3 and CHD4 Recruitment and Chromatin Remodeling Activity at DNA Breaks Is Promoted by Early Poly(ADP-Ribose)-Dependent Chromatin Relaxation.” Nucleic Acids Research 46(12):6087–98.

Cijsouw, Tony, Jens P. Weber, Jurjen H. Broeke, Jantine A. C. Broek, Desiree Schut, Tim Kroon, Ingrid Saarloos, Matthijs Verhage, and Ruud F. Toonen. 2014. “Munc18-1 Redistributes in Nerve Terminals in an Activity- and PKC-Dependent Manner.” Journal of Cell Biology 204(5):759–75.

Hägglund, Martin, Kimberly J. Dougherty, Lotta Borgius, Shigeyoshi Itohara, Takuji Iwasato, and Ole Kiehn. 2013. “Optogenetic Dissection Reveals Multiple Rhythmogenic Modules Underlying Locomotion.” Proceedings of the National Academy of Sciences of the United States of America 110(28):11589–94.

Chalmers, Susan, Stuart T. Caldwell, Caroline Quin, Tracy A. Prime, Andrew M. James, Andrew G. Cairns, Michael P. Murphy, John G. McCarron, and Richard C. Hartley. 2012. “Selective Uncoupling of Individual Mitochondria within a Cell Using a Mitochondria-Targeted Photoactivated Protonophore.” Journal of the American Chemical Society 134(2):758–61.

View publications



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