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Multi-Photon Microscopy

Photon-Based Deep Tissue Imaging

Multi-photon microscopy enables an increased penetration depth into the sample tissue by stimulating fluorescence in the near IR window of biological tissue, and by providing a distinct advantage in three-dimensional imaging. The low absorption of the stimulation light in the tissue avoids photobleaching and phototoxicity, while the multi-photon effect leads to a sharper definition of the focal plane, reducing background fluorescence. Multi-photon microscopy is the method of choice for three-dimensional imaging of living cell structures, such as brain slices, or in vivo embryos and adult animals.

The following multiphoton systems are available:

  • MOM® – Movable Objective Microscope system
  • DF-ScopeTM – Multi-photon imaging package for upright microscopes
Link to: MOM

MOM

Link to: DF-Scope

DF-Scope

Applications

  • In vivo multi-photon imaging
  • Electrophysiological recording and imaging (culture, large in vivo preparations, etc.)
  • Non-horizontal surface microscopy
  • Simultaneous retinal stimulation and two-photon microscopy
  • Whole animal imaging
  • Immunology
  • Embryology

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