Triple Emission Image Splitter
Cairn Research Ltd.
The OptoSplit III extends the OptoSplit image splitter concept, adding an optional second beamsplitter to split the field into either two or three separate, spatially equivalent, channels, which can be displayed side by side on a single camera sensor. The simple and accessible design makes the OptoSplit III an excellent platform for alternative applications, such as pFRET or multi-depth imaging.
Fast Simultaneous Imaging
Splitting is usually performed on the basis of wavelength or polarization, allowing applications with a requirement for simultaneous or high speed acquisition of multiple emission bands or polarization states. The simultaneous acquisition of up to three images offers a major benefit over manual or electronic filter changers, as there is no longer need to pause acquisition while the filter position is changed. This allows your camera to be operated in fast stream modes.
Device drivers are included in most commercial imaging packages to assist registration and to allow real-time and off-line ratioing of fluorescence overlays. Alternatively, the OptoSplit III can be used with simple image capture software, processing carried out manually offline or using our own MicroManager and ImageJ drivers.