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ACCUVA CELLECT

Laser Capture Microdissection (LCM)
LAXCO Inc.

The Accuva Cellect Laser Capture Microdissection (LCM) system is resulting from more than 20 years of experience in LCM. It combines the power of infrared (IR) laser capture and ultraviolet (UV) laser cutting in one unique, compact platform. The IR laser delivers a gentle capture technique that preserves the overall biomolecular integrity of cells, optimized for individual cells and small cell groups. The UV laser permits superior speed and precision and is well suited for microdissection of dense tissue structures and rapid capture of large numbers of cells.

Designed for laser capture microdissection, this dual-laser system ensures that sample custody is always maintained, thereby enabling researchers to uncover unique molecular signatures that would otherwise be obscured in a heterogeneous cell population.

The Accuva Cellect is designed and assembled by our Partner Laxco Inc. in the USA.

Features

Dual laser system

enables a wide range of applications from single cell isolation to large biopsy extractions. It expands utility and helps to ensure reliability of cell capture across different sample types without causing contamination or damage to morphology.

Minimized contamination

is ensured by the CapSure sample collection cap and overhead handling throughout the LCM process. The CapSure caps are moved by an automated system, reducing human interaction and minimizing potential contamination during cell collection.

Sample flexibility

is guaranteed as Accuva Cellect supports a wide range of slide types and sample preparation formats. Existing workflows can be used or validated workflows are available.

Intuitive software

allows rapid isolation of specific cells in five simple steps and quality control with visual inspection, making documentation and sample custody easy.

Compact design

is the result of the base unit’s footprint being around a third smaller than other microscope and laser systems, and the computer being built into the Accuva Cellect. This saves bench space.

Specifications

Sample types:

• Thin or thick sections

• Fresh frozen or freshly frozen, OCT-embedded (FFOE) tissue

• Formalin-fixed, paraffin-embedded (FFPE) tissues

• Fine-needle aspirates

• Chromogenic-stained, fluorescently stained, or unstained sections

• Hydrated or dehydrated specimens

• Smear preparations

• Live plant whole-mount preparations

• Live cell culture

Contrast methods:

• Brightfield

• Phase contrast

• Optional: Fluorescence (3 channels: DAPI, FITC, Texas Red)

Laser:  

• IR-Laser (808 nm)

• UV-Laser (355 nm)

LWD Objectives:

• 2x, 4x, 10x, 20x, 40x, 60x

Microscope stage:    

• Range: 155 x 125 mm

• Accuracy:  < 1 µm

• Software controlled

• Stage inserts for 3 traditional slides (75 x 26 mm), 2 large-format slides (75 x 50 mm) or 1 petri dish (50 x 7 mm)

Two cameras:

• Color

• Monochrome

Touchscreen:

UltraHD (27”)

Applications

• Mass spectroscopy
• NGS/ Gene expression
• DNA/RNA and protein analysis
• Oncology research
• Neuroscience research
• Plant biology
• Forensics and human identification (HID)
• Live cell microdissection
• Single-cell capture

Downloads

Product Documentation

  • Brochure Accuva Cellect (1.6 MB)
  • User Manual Accuva Cellect (3.1 MB)
  • Recommended consumables Accuva Cellect (197.9 KB)
  • Manufacturer website

Further Links

  • System introduction Accuva Cellect
  • Application Demo Accuva Cellect
  • Online Demo Accuva Cellect

Application Notes

  • Laser Capture Microdissection (LCM) for the Analysis of Macrophage Gene Expression Atherosclerotic Lesions (491.8 KB)
  • Laser Capture Microdissection of muscle fiber populations and expression analysis by RT-PCR (447.4 KB)
  • Laser Capture Microdissection of Cells Labeled with Enhanced Green Fluorescent Protein (322.4 KB)
  • Differential Gene Expression Analysis of Pure Cell Populations from Frozen Uterine Tissue Using Laser Capture Microdissection (LCM) and cDNA Microarrays (731.7 KB)
  • A Novel Process for Gene Expression Profiling of Rat and Mouse Tissues from Formalin-Fixed Paraffin-Embedded Sections Using Microarrays (1.4 MB)
  • Laser Capture Microdissection (LCM) and Expression Profiling of Long-Range Projection Neurons (871.1 KB)
  • Microgenomic Expression Profiling (757.4 KB)
  • Optimized Isolation of Live Plant Tissue using Laser Microdissection for use in Gene Expression Analysis (548.9 KB)
  • Genomic Analysis of Intestinal Responses to Gut Flora Altered by Antimicrobials in Human Flora-Associated (HFA) Mice (724.8 KB)
  • Optimized protocol for laser microdissection of living in-vitro cells (546.9 KB)
  • Microdissection to Microarrays: A Complete Solution for Gene Expression Profiling of Precious Tissue Samples (859.8 KB)
  • Isolation of Living in Vitro Cells Using the Accuva Cellect (791.2 KB)
  • From Postmortem Homogeneous Cells to Microarray Analysis: An Optimized Protocol (856.9 KB)



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