The dual emission image splitter OptoSplit II, a product of our partner company Cairn Research Ltd., is a simple device enabling a single camera to record images simultaneously at two different optical wavelengths, polarization states or other differentiated states. To improve the usability for multi-user microscopes, please have a look at the OptoSplit II with bypass option (OptoSplit II BP).
Fast and Low Cost Simultaneous Imaging
Traditionally, dual channel imaging is performed using an electronic filter changer or an additional camera and beam splitter, neither of which is ideal for all applications. The switching speed of an electronic filter changer limits the temporal resolution, whereas a second camera adds cost and complexity. In comparison, the OptoSplit II is a low cost option for simultaneous imaging without any time delays between the acquired channels.
Easy and Optimal Alignment
The OptoSplit II uses a unique rotating mirror cradle, which gives adjustable spatial separation to ensure excellent image registration. It features a fully adjustable rectangular aperture to enable cropped sensor imaging modes and reduced scatter. The adjustment is quite simple and allows to setup the module on your microscopic system by yourself.
For Large Camera Sensors
The latest version uses our own lens design to support larger camera sensors of scientific CMOS and EMCCD cameras. The instruments have a correspondingly larger aperture and improved off-axis correction to give enhanced performance with all camera sensors.
- Simultaneous multi fluorescent probe imaging
- Simultaneous phase contrast/ DIC and fluorescence
- Simultaneous multi depth imaging (using independent lenses)
- Förster Resonance Energy Transfer (FRET)
- Ratiometric calcium, voltage and pH-imaging (https://www.youtube.com/watch?v=GKNFYARHfcQ)
- Photoconversion/ Photoswitching
- Total Internal Reflection Fluorescence (TIRF)
- Spinning disk confocal microscopy
- Single Plane Illumination Microscopy (SPIM)
- Polarization studies (Anisotropy)
- 3D super resolution PALM/ STORM (using cylindrical lenses)
- Compact design with integral C-mount input and output ports
- Simple and precise controls for image registration
- Interchangeable filter/ dichroic holders
- Rotating filter mount for polarization studies
- 1x, 1.3x and 1.7x magnification available
- Support for sensors up to 18.8 mm diagonal (13.3 mm x 13.3 mm sensors)
- Angled and flat auxiliary drop ins for neutral density filtering, polarizers or chromatic correction
- Supports 25 mm filters
- Supports 2 mm thick Ultra-flat dichroics along with our recommended size (26x38x2 mm)
- 40 mm diameter proprietary optics
- 425 nm to 875 nm coatings on all surfaces
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