X-Light V2

Spinning Disk Confocal System
CrestOptics S.p.A.

The X-Light V2 spinning disk system from CrestOptics offers camera-based imaging with low phototoxicity for your daily research, where fast confocal imaging is required. The motorized movement of the single pinhole pattern in and out of the beampath allows a fast switch between confocal and widefield imaging. A special customized internal relay lens setup gives the possibility to image close to the diffraction limit across the whole field of view. A high confocal resolution, an improved out of focus rejection, and a higher signal-to-noise ratio is ensured by the CrestOptics proprietary disk pattern design enabling high quality fluorescence imaging.

Features

Compatibility

The X-Light V2 is compatible with all laser and high power LED light sources and can easily upgrade all inverted and upright research microscopes of the major brands. Furthermore, this confocal system matches with external automated filter wheels and optical splitters such as the multi-camera adapters and the image splitters from Cairn Research Ltd..

Flexibility

The pinhole disk itself is mounted in a box allowing an easy startup and exchange in a few seconds by plug-and-play. No alignment is required and the system can be used directly after the exchange. In addition, CrestOptics can design your specific custom spiral pinhole pattern on request. For further information, please get in contact with us.

In case a high power multi-LED or multi-laser light source is adapted, multi-wavelength imaging of up to four channels is possible simultaneously, supported by the multi-position excitation, the emission and dichroic motorized filter wheels.

Easy Handling

The X-Light V2 can be easily installed and aligned. For example, the detector focal plane will be focused without moving the detector. The excitation Gimbal mount ensures a fast and easy alignment of the optical fiber and guaranties the best signal-to-noise ratio as possible. Additionally, the delivery content includes extraction tools for easy insertion and removal of both dichroic filter and emission filter. As a conclusion, the X-Light V2 spinning disk system is a flexible and user-friendly confocal system to setup your fully automated experiments for your daily research.

Applications

Specifications

Compatible microscopes:	All inverted and upright microscopes (100% C-mount)
Connection port:	Camera port (1x C-mount adapter required)
Light source:	Laser or high power LED
Light source connector:	SMA
Optical fiber:	Multimode; 0,39 NA; diameter: 1,5 mm
Spectral range:	400 - 750 nm
Pinhole size:	50 µm
(or customized pinhole size spiral pattern on request)	(or customized pinhole size spiral pattern on request)
Camera ports:	1
Compatible cameras:	CCD, EMCCD, sCMOS (up to 1”)
Field number:	Up to 22
Lateral Resolution (FWHM):	~ 230 nm (objective: 60x NA 1.4 oil); diffraction limited
Axial Resolution (FWHM):	~ 600 nm (objective: 60x NA 1.4 oil)
Rotation speed:	Up to 15.000 RPM
Acquisition speed:	Up to 1000 fps
Bypass:	motorized
Excitation and emission filter wheel:	Motorized, 8 positions (each)
Dichroic filter wheel:	Motorized, 5 positions
Excitation and emission filter dimensions:	25mm diameter, up to 5mm thickness
Dichroic dimensions:	25.5 mm x 36 mm x 1-2 mm; ultraflat
Connection port:	USB serial
Software:	MicroManager, MetaMorph, NisElements

Downloads

Literature

Zubkovs V, Antonucci A, Schuergers N, Lambert B, Latini A, Ceccarelli R, Santinelli A, Rogov A, Ciepielewski D and Boghossian A A (2018) Spinning-disc confocal microscopy in the second near-infrared window (NIR-II). Scientific Reportsvolume 8, Article number: 13770

Filipis L., Ait Ouares K, Moreau P, Tanese D, Zampini V, Latini A, Bleau C, Bleau C, Graham J and Canepari M (2017) A novel multisite confocal system for rapid Ca2+ imaging from submicron structures in brain slices. J. Biophotonics, e201700197. Accepted Author Manuscript. doi:10.1002/jbio.201700197

Cegla J, Jones B J, Gardiner J V, Hodson D J, Marjot T, McGlone E R, Tan T M and Bloom S R (2017) RAMP2 Influences Glucagon Receptor Pharmacology via Trafficking and Signaling. Endocrinology, Volume 158, Issue 8; Pages 2680–2693

Kozaki I, Shimizu K and Honda H (2017) Effective modification of cell death-inducing intracellular peptides by means of a photo-cleavable peptide array-based screening system. Journal of Bioscience and bioengineering; 124(2):209-214

Quaranta R, Pelullo M, Zema S, Nardozza F1, Checquolo S, Lauer DM, Bufalieri F, Palermo R, Felli MP, Vacca A, Talora C, Di Marcotullio L, Screpanti I and Bellavia D (2017) Maml1 acts cooperatively with Gli proteins to regulate sonic hedgehog signaling pathway. Cell Death and Disease 8(7): e2942

Tkatch T , Greotti E , Baranauskas G , Pendin D , Roy S , Nita LI , Wettmarshausen J , Prigge M , Yizhar O , Shirihai OS , Fishman D , Hershfinkel M, Fleidervish IA , Perocchi F, Pozzan T and Sekler I (2017) Optogenetic control of mitochondrial metabolism and Ca2+ signaling by mitochondria-targeted opsins. Proc Natl Acad Sci U S A. 2017 Jun 27;114(26):E5167-E5176. doi: 10.1073/pnas.1703623114.