MOM

Movable Objective Microscope
Sutter Instrument

The Movable Objective Microscope® (MOM®) is a two-photon microscope capable of imaging deep within living specimens when combined with a Ti:Sapphire Laser. The MOM design is unique in providing three-dimensional objective movement and rotation allowing the specimen to remain stationary. Many highly regarded imaging laboratories around the world use the Sutter MOM and we constantly work with our customers to adapt the design for their changing needs.

Opto-mechanical Design

Opto-mechanical Design

The MOM consists of two independent microscopes. The wide-field half of the microscope consists of an Olympus vertical illuminator, a LED light source and camera mount to provide standard epifluorescence. The two-photon side of the microscope provides the optical pathway for guiding the excitation laser light from the table up into the scanning mirrors. From there the beam expands through the scan lens and directs into the back of the objective. Following two-photon excitation, the emitted photons are directed into the detection pathway by a dichroic mirror immediately above the objective. The main body of the microscope moves backwards on a rail system allowing easy access to the specimen prior to imaging.

The objective translates in X, Y and Z axes, it additionally rotates around the Y axis. Two moving mirrors allow the microscope to maintain efficient delivery of the excitation light to the back aperture of the objective regardless of movement or orientation. The X, Y and Z movements used are the same as controlled in Sutter’s MP-285 micromanipulator, thus movements are smooth, fine in scale, drift-free and highly reproducible. These movements allow the acquisition of Z-stacks and mosaic images of large regions of tissue without the need for a moving stage.

The horizontal light path allows for rotation of the objective away from the standard vertical position. As a result of this rotation, the MOM can easily be converted from an upright to an inverted microscope and the objective positioned from 0 to 180 degrees. This positional freedom permits the imaging of non-horizontal surfaces and volumes.

Scanning Systems

Scanning Systems

During the last 10 years, scanning technology has changed dramatically: objectives changed requiring larger beam sizes and scanner technology advanced providing dependable resonant scanners. Unlike other two-photon microscope designs, the MOM has lived through and adapted to changes in scanning technology. Throughout this development, Sutter has maintained two principles. First, existing scopes can be upgraded to new technology as it becomes available. Many original scopes with 3 mm galvo scanners have been upgraded to either 5 or 6 mm galvo scanners or resonant/ galvo scanners. Second, Sutter continues to supply the original designs if they are needed for current research.
We can provide a 5 mm conventional galvo-galvo scanning (GG) MOM, resonant-scanning (RG), or resonant-galvo-galvo scanning (RGG) MOM, all at competitive prices.

The MOM is available with four different detector path designs. The original two channel pentagon, which can become a four channel detector path. The Short path and the Wide path are two designs that increase the chances of capturing ballistic photons by getting the first collection lens either closer to the back aperture of the objective (Short path) or by using a larger aperture collection lens and dichroic (Wide path).

Sutter MOM packages include all of the equipment (less the Femtosecond laser and objective) needed for a complete imaging system.

Scanner systems either GG, RG or RGG system design with accompanying controller
Hamamatsu photomultiplier tubes (PMTs):
multialkali or GaAsP from standard to gated products
(Sutter is an authorized reseller for Hamamatsu)
Power supplies for PMTs:
Either a Sutter PS-2 (dual channel high-voltage power supply for multialkali PMTs) or
Sutter PS-2LV (dual channel low-voltage power supply for GaAsP PMTs) can be ordered
Hamamatsu, Sigmann or FEMTO pre-amplifiers, selection varies with software and type of scan
Data acquisition: MBF Bioscience vDAQ or National Instruments

Features

Objective moves 22 mm in X, Y and Z
Objective rotates about optical axis for imaging of non-horizontal surfaces and volumes
Customizable open platform design
conventional GG, RG or RGG scanners
Two or four channel detector system with Hamamatsu PMTs and preamplifiers
Sutter PS-2/ PS-2LV dual channel PMT power supply
MBF Bioscience or National Instruments data acquisition systems
MBF Bioscience microscope control software ScanImage basic or premium

Applications

Software

The MOM® has always been compatible with ScanImage, the two-photon imaging software based on a Matlab (the MathWorks) environment developed by Karel Svoboda and collaborators. One of the reasons the MOM platform exists in its present form is the strong support from the ScanImage community. In 2014, Vidrio became the primary vendor for support and new development of ScanImage. In 2021 Vidrio got acquired by MBF Bioscience and benefits from the long lasting software development of their combination.

Rapp is happy to make ScanImage Basic and Premium available to customers who wish premium support and the latest features. ScanImage freeware is also still available as SI5. Rapp provides packages that include the necessary data acquisition hardware to couple the MOM and other scanning microscopes to ScanImage Basic, Premium or SI5.

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