DF-Scope

(Olympus microscope not included)

The DF-Scope™ is a customer-inspired, multi-photon, imaging package for the ubiquitous BX51WI upright microscope. Many laboratories already use BX51WI microscopes for electrophysiology and epifluorescent imaging experiments. The DF-Scope package provides the necessary optics and electronics for the BX51WI to be used for multi-photon imaging (with the addition of a Ti:Sapphire laser). The design incorporates subassemblies from our MOM™ (Movable Objective Microscope®) system including resonant and galvo scan boxes and controllers, detector paths, PMTs, PMT power supplies, scan lenses and tube lenses.

A hallmark of multi-photon imaging is that all of the light emitted by the sample is known to emit from the focal volume as a result of the non-linear excitation of the fluorophore. High sensitivity photomultiplier tubes (PMTs) are used to collect as many photons as possible in order to reconstruct the scanned image. The DF-Scope design allows for two detector paths to gather more emitted light – one above the sample and one below. If using a thin sample, like a brain slice, we recommend the lower (sub-stage) detectors for increased signal detection. Additional signal will be available at the trans detector path. This substage detector assembly is designed to work with a variety of Olympus condensers.

NOTE: The DF-Scope design requires an Olympus BX51WI and the following Olympus parts: WI-ARMAD, 5-UR710LP and U-M619.