(Olympus microscope not included)
The DF-Scope™ is a customer-inspired, multi-photon, imaging package for the ubiquitous BX51WI upright microscope. Many laboratories already use BX51WI microscopes for electrophysiology and epifluorescent imaging experiments. The DF-Scope package provides the necessary optics and electronics for the BX51WI to be used for multi-photon imaging (with the addition of a Ti:Sapphire laser). The design incorporates subassemblies from our MOM™ (Movable Objective Microscope®) system including resonant and galvo scan boxes and controllers, detector paths, PMTs, PMT power supplies, scan lenses and tube lenses.
A hallmark of multi-photon imaging is that all of the light emitted by the sample is known to emit from the focal volume as a result of the non-linear excitation of the fluorophore. High sensitivity photomultiplier tubes (PMTs) are used to collect as many photons as possible in order to reconstruct the scanned image. The DF-Scope design allows for two detector paths to gather more emitted light – one above the sample and one below. If using a thin sample, like a brain slice, we recommend the lower (sub-stage) detectors for increased signal detection. Additional signal will be available at the trans detector path. This substage detector assembly is designed to work with a variety of Olympus condensers.
NOTE: The DF-Scope design requires an Olympus BX51WI and the following Olympus parts: WI-ARMAD, 5-UR710LP and U-M619.
- Converts a standard Olympus BX51WI into a two-photon microscope while retaining standard microscope functions (transmitted light and epifluorescent imaging).
- Includes detector(s) and “whisperquiet” resonant scan box developed for the MOM™ Two-Photon microscope
- Upper and lower photodetectors for increased collection efficiency
- Fully compatible with the Sutter MPC-78 Large Moving Stage Platform and motorized focus drive
- Designed to be controlled with the Sutter MCS (MOM Computer System) Imaging Software including resonant scanning with MScan 2.0
- Also fully compatible with most multi-photon freeware such as ScanImage 5.0, Helioscan, and MPScope
- Breadboard format in scan pathway allows easy addition of photostimulation light sources to the main scanned laser path
- In vivo and in vitro two-photon imaging
- Whole-cell imaging
- Intracellular imaging