Multi-Photon Microscopy

Multi-photon microscopy enables an increased penetration depth into the sample tissue by stimulating fluorescence in the near IR window of biological tissue, and by providing a distinct advantage in three-dimensional imaging. The low absorption of the stimulation light in the tissue avoids photobleaching and phototoxicity, while the multi-photon effect leads to a sharper definition of the focal plane, reducing background fluorescence. Multi-photon microscopy is the method of choice for three-dimensional imaging of living cell structures, such as brain slices, or in vivo embryos and adult animals.