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NWG Göttingen 2019
13th meeting of the German Neuroscience Society

Mar 20-23, 2019, Göttingen, Germany
Booth No. 8

 

SFN - Neuroscience 2019
50th annual meeting of the Society for Neuroscience

Oct 19-23, 2019, Chicago, IL, USA
Booth No. 1468

 

BPS San Diego 2020
64th annual meeting of the Biophysical Society

Feb 15-19, 2020 San Diego, CA, USA
Booth No. 710

Localized Glutamate Uncaging in CA1 Pyramidal Neurons in Brain Slices

EPSCs (Excitatory Post Synaptic Currents) obtained in whole-cell voltage clamp mode in response to focal uncaging of glutamate onto multiple locations (marked by red crosses) around a single dendritic branch of a CA1 pyramidal neuron. Neurons were loaded with bis-Fura-2 by intracellular injection in a brain slice. The release zone is highly localized as evident from the relationship between the signal amplitude and the distance of the uncaging spot from the dendrite. (Uncaging in seven spots between the arrow heads)

 

Equipment:

 

  • Rapp OptoElectronic UGA-40 spot illumination system with DL-405/100 laser on Zeiss AxioObserver, 63x water immersion objective, RedShirt Imaging camera.
  • Spot size <1µ.
  • Synchronization via Master8 TTL protocols
Excitatory Post Synaptic Currents evoked by localized Glu uncaging in and near dendrites

Data courtesy of Dr. Marko Popovic, Laboratory of Dr. Dejan Zecevic, Dept. of Cellular & Molecular Physiology, Yale School of Medicine, New Haven, USA.

 
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