Rapp OptoElectronic
  • Home
  • Products
    • Photomanipulation & Microdissection (LCM)
      • Scanner-Based Photomanipulation – UGA-42 Series
        • UGA-42 Firefly
        • UGA-42 Geo
        • UGA-42 Caliburn
      • Fixed-Spot Photomanipulation – AiWon Series
        • AiWon
        • AiWon Geo
        • AiWon Caliburn
      • Advanced Photomanipulation
        • FLUCS Micro Flow Photomanipulation
        • HOLO4D Holographic Photomanipulation
      • Laser Capture Microdissection (LCM) Accuva Cellect
    • Microscopy & Imaging
      • Multi-Photon Microscopy
        • MOM
        • DF-Scope
      • Raman Microscopy
        • XperRAM C Series
      • Deep UV Microscopy
      • Spinning Disk Microscopy
        • X-Light V2
      • Further Microscopy Systems
        • BOB
        • SOM
        • OpenFrame
    • Microscope Accessories
      • Optical Couplings
        • IX3-Breadboard
        • FC-70 Series
        • LGA
      • Camera Adapter
        • IX3-CAM Adapter
        • TwinCam
        • MultiCam
      • Image Splitter
        • OptoSplit II
        • OptoSplit II BP
        • OptoSplit III
        • MultiSplit V2
    • Illumination Systems
      • LED & Bulb Systems
        • KSL2
        • UVILED
        • UVICO-2
        • FuraLED
        • OptoScan
      • Laser Systems
        • Single Line Laser
        • Laser Combiner
      • Flash Lamp Systems
        • JML-C2
        • SP-20
  • Applications
    • Ablation & Microdissection
    • DNA Damage
    • IR Temperature Jump
    • Uncaging & Photolysis
    • Optogenetics & Photostimulation
    • Photobleaching & FRAP
    • Photoconversion & Photoswitching
  • Publications
    • Ablation & Microdissection
    • DNA Damage
    • IR Temperature Jump
    • Uncaging & Photolysis
    • Optogenetics & Photostimulation
    • Photobleaching & FRAP
    • Multi-Photon Imaging
    • Microscope Illumination
    • FLUCS Micro Flow
  • About us
    • Partners
    • Team
    • Our story
    • Location
  • Events
  • Contact
  • Career
  • Search
  • Menu Menu

OptoSplit II BP

Dual Emission Image Splitter with Bypass
Cairn Research Ltd.

The dual emission image splitter OptoSplit II Bypass (BP) from our partner company Cairn Research Ltd. is the improved dual channel simultaneous imaging device for use with a single camera. It builds on the success of the OptoSplit II, but adds a convenient single lever bypass mode making it more suitable for multi-user microscopes where simultaneous dual channels are only required for specific experiments alongside single wavelength recordings. In addition, the simple and accessible design makes the OptoSplit II BP an excellent platform for alternative applications, such as dual polarization imaging.

Features

High-End Dual Image Splitter

Whilst maintaining compatibility with the OptoSplit II, the bypass version now supports our new flat-face filter cubes and has enhanced long-term stability, pixel registration and reproducibility. Feature wise, the rapid bypass control is complimented by additional space for more auxiliary components. It has a slightly larger footprint than the OptoSplit II and consequently, can use longer focal ratio lenses with even better off-axis performance. Optimized for coupling to a scientific microscope, the OptoSplit II BP can also be used with camera lenses or any other system of lenses which produce an image plane of suitable size and f/number.

Software Implementation

Device drivers are included in most commercial imaging packages to assist registration and to allow real-time and off-line ratioing of fluorescence overlays. Alternatively, the OptoSplit II BP can be used with simple image capture software, the processing carried out manually offline or by using our own MicroManager and ImageJ drivers.

Applications
  • Simultaneous multi fluorescent probe imaging
  • Simultaneous phase contrast/ DIC and fluorescence
  • Simultaneous multi depth imaging (using independent lenses)
  • Förster Resonance Energy Transfer (FRET)
  • Ratiometric calcium, voltage and pH-imaging
  • Photoconversion/ Photoswitching
  • Total Internal Reflection Fluorescence (TIRF)
  • Spinning disk confocal microscopy
  • Single Plane Illumination Microscopy (SPIM)
  • Polarization studies (Anisotropy)
  • 3D super resolution PALM/ STORM (using cylindrical lenses)
Specifications
  • Compact design with integral C-mount input and output ports (optional T- or F-mount)
  • Simple and precise controls for image registration
  • Interchangeable filter/ dichroic holders
  • Single lever switching from split to bypass
  • Rotating filter mount for polarization studies
  • 1x or 1.3x magnification
  • Support for sensors up to 18.8 mm diagonal (13.3 mm x 13.3 mm sensors)
  • Angled and flat auxiliary drop in for neutral density filtering, polarizers or chromatic correction
  • Supports 25 mm filters and standard microscope dichroic sizes
  • 40 mm diameter optics
  • 425 nm to 875 nm coatings on all surfaces
  • Fixed or variable center fully adjustable rectangular mask to delimit region of interest
Downloads
  • OptoSplit II BP Flyer (603.0 KB)
  • OptoSplit II BP Manual (1.8 MB)
  • Support video (Intro):
https://www.youtube.com/watch?v=vt0ZSCDqSvk



Copyright © 2025
  • Contact
  • Newsletter
  • Imprint
  • Privacy Policy
  • Sitemap
Scroll to top