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AiWon Geo

Selectable Shape Illumination Device for Localized Photomanipulation

The AiWon Geo is another member of our AiWon series enabling a selectable shape illumination for localized photomanipulation. In contrast to the AiWon itself, the AiWon Geo was mainly designed to generate areas of different size and shape in the center of the field of view, while the position of the shape is fixed. Thus, the AiWon Geo is the perfect system for applications where simultaneous illumination of a selected  area is crucial (e.g. FRAP of fast diffusing molecules, optogenetic stimulation).

Features

Shape Illumination/ Simultaneous Illumination of Large Areas

The AiWon Geo comes with more than 40 hardware-defined geometries like circles, squares, rectangles and donuts of different size. By reshaping the laser spot, simultaneous illumination of the entire geometry is enabled. It allows illumination of larger ROIs such as entire cells without temporal delay.

Modularity and Flexibility

The AiWon Geo can be upgraded with the AiWon Caliburn ablation system and additional ports for light sources. Instead of a single line laser light source, a laser combiner like the LDI laser diode illuminator can be coupled to the AiWon Geo module to provide even more flexibility for your application.

Applications
  • Photoactivation/ Photoinhibition
  • Photoconversion/ Photoswitching
  • Photoexcitation
  • Photobleaching/ FRAP
  • Uncaging/ Photolysis
  • Optogenetics/ Photostimulation
  • Neural mapping, dynamics and plasticity
  • Infrared Laser Temperature Jump
Downloads

For microscopes:

  • Flyer Photomanipulation (3.5 MB)
Literature

Hirsch, Sophia M., Sriramkumar Sundaramoorthy, Tim Davies, Yelena Zhuravlev, Jennifer C. Waters, Mimi Shirasu-Hiza, Julien Dumont, and Julie C. Canman. 2018. “FLIRT: Fast Local Infrared Thermogenetics for Subcellular Control of Protein Function.” Nature Methods.

Sundaramoorthy, Sriramkumar, Adrian Garcia Badaracco, Sophia M. Hirsch, Jun Hong Park, Tim Davies, Julien Dumont, Mimi Shirasu-Hiza, Andrew C. Kummel, and Julie C. Canman. 2017. “Low Efficiency Upconversion Nanoparticles for High-Resolution Coalignment of Near-Infrared and Visible Light Paths on a Light Microscope.” ACS Applied Materials & Interfaces 9(9):7929–40.

Suzuki, Motoshi, Yasuhiro Kamei, Shunsuke Yuba, and Shin Takagi. 2009. “Manipulating Behaviors of Targeted Single Cells in Vivo by Using IR-LEGO.” 20th Anniversary MHS 2009 and Micro-Nano Global COE – 2009 International Symposium on Micro-NanoMechatronics and Human Science 326–31.

View publications

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