The Holo4D system is a holographic illumination system designed for simultaneous illumination of arbitrary spot patterns and shapes in 3D using two-photon-stimulation. The holographic approach uses a spatial light modulator allowing the generation of arbitrary 3D light fields while maintaining a high light utilization efficiency.
The Holo4D includes the holographic spatial light modulator module, optical and mechanical components necessary to attach the module to the microscope, the microcontroller and the software to create spots. Standard systems provide the possibility to control up to four analogue or digital devices, e.g. a pockels cell for fast laser intensity regulation. The pockels cell is application specific and has to be ordered separately, as well as the laser, which is used together with this unit.
The Holo4D system is an add-on module and can be integrated into existing microscope setups like widefield fluorescence, LSM, spinning disc confocal, etc.. Modules are available for most modern research microscope brands and models. Furthermore, the system can be integrated with any existing NIR laser source, providing sufficient power for obtaining two-photon effects. To control the system, the Rapp OptoElectronic software SysCon can be used, or the TTL ports for triggered interaction with existing imaging or electrophysiology equipment shall be used.
- Uncaging/ Photolysis
- Optogenetics/ Photostimualtion
- Photoconversion/ Photoswitching
- Neural mapping, dynamics and plasticity
- Dendritic integration
|Compatible microscopes:||Most Zeiss, Olympus, Leica and Nikon systems; Multi-photon systems (others on request)|
|Addressable sample volume (W x D x H):||more than 150 x 150 x 400 μm (standard 20x objective)|
|Update rate:||60 Hz|
|Spot size:||< 1 µm (depends on microscope and objective)|
|Synchronization with external devices:||2x TTL out (BNC); 2x TTL in (BNC)|
|Laser or pockels cell modulation digital (TTL):||4x Outputs (RMI)|
|Laser or pockels cell modulation analog (+/- 5V):||4x Outputs (RMI)|
|Time resolution laser control via pockels cell:||1 µs|