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UGA-42 Geo

Selectable Shape Scanning Device for Localized Photomanipulation

The UGA‑42 Geo is another member of the UGA‑42 series of laser scanner devices for localized photomanipulation. In contrast to the UGA‑42 Firefly, the UGA‑42 Geo is mainly designed to generate illuminated regions of different size and shape in the field of view of the microscope. These selected regions can be rapidly moved with the galvanometric mirrors on the field of view. Thus, the UGA‑42 Geo is the perfect system for applications where simultaneous illumination of an entire area is crucial, e.g. in optogenetics where the time needed to scan a region of interest (ROI) might take too long to open a certain number of channels. The optical module is coupled into the backport to the microscope without interfering with most other existing devices (e.g. spinning disk, LSM, etc.).

Features

Shape Illumination/ Simultaneous Illumination of Large Areas

The UGA‑42 Geo comes with preset hardware-defined geometries like circles, squares, rectangles and donuts of different size. By reshaping the laser spot, simultaneous illumination of a selected geometry is enabled. It replaces the necessity to scan larger ROIs such as entire cells, and avoids a temporal delay during the illumination.

Modularity and Flexibility

The UGA‑42 Geo includes the UGA-42 Firefly which allows free positioning of the chosen shape within the field of view. This increases the flexibility for your application and system. In addition, the UGA‑42 Geo can be combined with the UGA‑42 Caliburn ablation system. Furthermore, in addition to a single line laser light source, a laser combiner like the LDI laser diode illuminator can be coupled to the UGA‑42 Geo module to provide even more flexibility for your present and future experimental needs.

Applications
  • Photoactivation/ Photoinhibition
  • Photoconversion/ Photoswitching
  • Photoexcitation
  • Photobleaching/ FRAP
  • Uncaging/ Photolysis
  • Optogenetics/ Photostimulation
  • Neural mapping, dynamics and plasticity
  • Infrared Laser Temperature Jump
Downloads

For microscopes:

  • Flyer Photomanipulation (3.5 MB)

For macroscopes:

  • Flyer UGA-42 series for Olympus MVX10 (1.1 MB)
  • Flyer UGA-42 series for Zeiss AxioZoom.V16 (1.1 MB)

SysCon Software:

  • Flyer SysCon & ZEN Communication (1.1 MB)
  • Flyer SysCon & NIS Elements Communication (1.1 MB)
  • Flyer SysCon & MicroManager Communication (1.1 MB)
  • Flyer SysCon & MetaMorph Communication (1.1 MB)
  • Flyer SysCon Software & Communication Plugin (1.2 MB)
  • Flyer SysCon & cellSens Communication (1.2 MB)
Literature

Hirsch, Sophia M., Sriramkumar Sundaramoorthy, Tim Davies, Yelena Zhuravlev, Jennifer C. Waters, Mimi Shirasu-Hiza, Julien Dumont, and Julie C. Canman. 2018. “FLIRT: Fast Local Infrared Thermogenetics for Subcellular Control of Protein Function.” Nature Methods.

Sundaramoorthy, Sriramkumar, Adrian Garcia Badaracco, Sophia M. Hirsch, Jun Hong Park, Tim Davies, Julien Dumont, Mimi Shirasu-Hiza, Andrew C. Kummel, and Julie C. Canman. 2017. “Low Efficiency Upconversion Nanoparticles for High-Resolution Coalignment of Near-Infrared and Visible Light Paths on a Light Microscope.” ACS Applied Materials & Interfaces 9(9):7929–40.

Suzuki, Motoshi, Yasuhiro Kamei, Shunsuke Yuba, and Shin Takagi. 2009. “Manipulating Behaviors of Targeted Single Cells in Vivo by Using IR-LEGO.” 20th Anniversary MHS 2009 and Micro-Nano Global COE – 2009 International Symposium on Micro-NanoMechatronics and Human Science 326–31.

View publications



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